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The Cryo-EM Center at UB-HWI houses a Thermo Fisher Glacios Cryo-TEM operating at 200kV with a Falcon 4 direct electron detector. In addition to cryo-TEM imaging, we routinely perform single particle analysis, tomography, and microcrystal electron diffraction (microED) experiments. A Thermo Fisher Vitrobot is available for sample preparation by plunge-freezing, and a multi-GPU cluster allows on-the-fly data processing to assess sample quality, as well as in-depth data processing. Expert assistance is provided for all steps from experiment design to data processing. The center is open for use by the UB community, external academic groups, and external industry researchers.
Katherine Spoth - Technician
Michael Rhodes - Administrator
| Hours | Location |
|
Monday - Friday 8:30 am - 4:30 pm |
University at Buffalo Hauptman Woodward Research Institute 700 Ellicott Street Buffalo, NY 14203 |
To learn more about the Cryo-EM service center, Click Here.
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Katherine Spoth, PhD |
Technician
|
716-881-8210
|
kspoth@buffalo.edu
|
700 Ellicott Street, Buffalo, NY 14203
|
| Michael Rhodes |
Administrator
|
716-881-8208
|
mjrhodes@buffalo.edu
|
700 Ellicott Street, Buffalo, NY 14203
|
| Service list |
| Name | Description | Price |
|---|---|---|
| 15-hour overnight collection (automated) |
Time on Glacios cryo-TEM for overnight automated data collection after a daytime screening session. |
Inquire |
| 23-hour data collection (automated) |
Time on Glacios Cryo-TEM for automated data collection lasting one day. |
Inquire |
| 7-hour daytime session with staff operator |
Daytime session on Glacios cryo-TEM for grid screening and imaging with staff assistance. |
Inquire |
| C-Clip and C-Clip Ring Pair |
Rigid support for TEM grid so it can be handled by the autoloader; required for any grid to be loaded into the microscope |
Inquire |
| Cryo-Grid Box |
Box to store 4 grids in liquid nitrogen before or after imaging in the TEM |
Inquire |
| Data analysis |
Hourly charge for staff time spent processing cryo-EM data, including: 1. Single-particle processing to produce 2D classes for sample quality analysis or a 3D density map of particle of interest 2. Tomography data processing to produce reconstructed 3D volume from tilt series 3. Other image processing as needed |
Inquire |
| Grid |
300-mesh copper TEM grid with R1.2/1.3 Quantifoil carbon film, specimen carrier for loading frozen sample into the microscope |
Inquire |
| Thermo Fisher Glacios Cryo-TEM |
Hourly use of Glacios Cryo-TEM with staff operator. |
Inquire |
| Thermo Fisher Vitrobot |
Grid preparation by plunge freezing using Thermo Fisher Vitrobot. |
Inquire |
